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Comparative Sensitivities and Specificities of Antibodies to Breast Markers GCDFP-15, Mammaglobin A, and Different Clones of Antibodies to GATA-3: A Study of 338 Tumors Using Whole Sections

发布日期:2017/2/25 0:44:43 文章来源: 作者: 点击次数:8932

 

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Appl Immunohistochem Mol Morphol. 2016 Oct;24(9):609-614.

Comparative Sensitivities and Specificities of Antibodies to Breast Markers GCDFP-15, Mammaglobin A, and Different Clones of Antibodies to GATA-3: A Study of 338 Tumors Using Whole Sections

Kandalaft PL1, Simon RA, Isacson C, Gown AM.

1*The PhenoPath Laboratories PLLC †Pacific Pathology Partners ‡CellNetix Pathology and Laboratories, Seattle, WA.

 

Abstract

 GATA-3 is a transcription factor that has recently been identified by immunohistochemistry to be highly expressed in urothelial and breast carcinomas (CAs). We sought to determine the potential utility of GATA-3 in identifying metastatic breast CA, and to compare its utility with the standard breast markers, GCDFP-15, and mammaglobin A. We identified an archival series of 338 formalin-fixed paraffin-embedded whole-tissue sections of various CAs. Using standard immunohistochemical (IHC) techniques we used mouse monoclonal antibodies to GATA-3 (clones L50-823, HG3-31), GCDFP-15 (23A3), and mammaglobin A (31A5). Both clones of GATA-3 showed positivity in 96% of non–triple-negative breast carcinomas (TNBCs), L50-823 and HG3-31, demonstrating expression in 87% and 63% of TNBCs, respectively; GCDFP-15 and mammaglobin A were expressed in 69% and 61% of non-TNBCs, respectively, and 10% and 17%, of TNBCs, respectively. The L50-823 clone manifested a lower specificity in identifying breast CAs (84%) than did the HG3-31 clone (97%). Both monoclonal antibodies to GATA-3 are very sensitive reagents for the identification of breast CA, surpassing antibodies to GCDFP-15 and mammaglobin A, and offer a significant improvement in identifying TNBCs. However, the L50-823 clone showed a lower level of specificity, which may qualify its utility in the setting of CAs of unknown primary.

 

乳腺免疫组化标记物GCDFP-15, Mammaglobin A以及GATA-3抗体不同克隆号敏感性和特异性的比较:338个病例的研究

摘要:最近通过免疫组织化学确定,GATA-3是一种在尿路上皮和乳腺癌中高度表达的转录因子。我们试图确定GATA-3在鉴别转移性乳腺癌中的潜在用途,并比较其与乳腺标志物GCDFP-15mammaglobin A的效用。本文的组织切片是用福尔马林固定石蜡包埋,包括各种乳腺癌病例,共338例。我们用标准免疫组织化学技术, GATA-3(克隆号L50-823HG3-31),GCDFP-1523A3)和mammaglobin A31A5)的小鼠单克隆抗体。GATA-3两种克隆抗体L50-823 HG3-31,在 96%的非三阴性乳腺癌均表达,而在非三阴性乳腺癌中的阳性率分别为:87%和63%。GCDFP-15mammaglobin A在非三阴性乳腺癌的阳性率为:69%和61%,在三阴性乳腺癌中的阳性率为:10%17%GATA-3(克隆号L50-823)在乳腺癌的的阳性率为84%,低于克隆号HG3-31 的阳性率,97%GATA-3这两种克隆号的抗体在鉴别乳腺癌中是非常灵敏的试剂,这点优于GCDFP-15mammaglobin A抗体,同时对于三阴性乳腺癌的鉴别也非常有用。但是,克隆号L50-823的特异性较低,限制了其在乳腺癌中的应用。(翻译:徐婉妮)

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